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1e), subsequent experiments were conducted using a lower dose (300 mg/kg)

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Chlamydial PCR DNA was extracted from whole blood 2, 3 and 4 days after infection using the Qiagen DNeasy blood and tissue kit according the manufacturers instructions

The flow rate was set at 0.4 mLmin 1 with the elution gradient as follows: 0-1.5 min, mobile phase B was increased from 5% to 30%

co-stimulatory ligand includes a molecule on an antigen presenting cell e.g., an APC, dendritic cell, B cell, and the like) that specifically binds a cognate co-stimulatory molecule on a T cell, thereby providing a signal which, in addition to the primary signal provided by, for instance, binding of a TCR/CD3 complex with an MHC molecule loaded with peptide, mediates a T cell response, including, but not limited to, proliferation, activation, differentiation, and the like