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Cell extracts were centrifuged at 3,000 g at 4C for 5 min

She has created a registry for physicians to report delayed rashes and is working on one for patients, in order to understand the range of what they can look like and detect any patterns about which rashes might be more worrisome

Cells were assigned to G1, S, and G2 phase based on PI fluorescence histograms analyzed with the FlowJo v10.8.1 cell cycle analysis tool and Dean-Jett-Fox model as described ( Western blotting All cells were seeded in 6-well plates to obtain 70-80% confluence at time of harvest

p-tert-Butylcalix[4]arene Functionalised with Bipyridyl Carboxylates for Lanthanide Complexation: Synthesis, Photophysical Properties Supramolecular chemistry, 2003, 15, 277289

94 Exposures to these heavy metals were subtoxic and not associated with hepatotoxicity, changes in behaviour, organ and body weights, food intake, stool consistency or gut motility