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Description

Figure 1 The upstream and downstream homologous arms of gr were amplified by PCR using primer pairs of gr UF/ gr UR [819 bp upstream homologous arm (UHA)] and gr DF/ gr DR [933 bp downstream homologous arm (DHA)]

The kanamycin cassette was removed from the resulting strains using the pCP20 helper plasmid [19] with the formation of the corresponding deletions ( cysB, cysE, gshA, gshB and emaA )