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The tetrapeptide (Nle1-YIH) and tripeptide (Nle1-YI) fragments of the Nle1-AngIV analog of AngIV were previously found to be the smallest active fragments capable of overcoming scopolamine-induced cognitive dysfunction in a spatial learning task

For the FP competition assay, the unlabelled compounds (from 50-mM DMSO stocks) were first dispensed in duplicate into wells of a Proxiplate 384 F Plus plate using an Echo 550 acoustic liquid handler to give a 13-point concentration range from 0.2 to 500 M

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Alonso-Alvarez S, Pardal E, Sanchez-Nieto D, Navarro M, Caballero MD, Mateos MV, et al

Missing data for the trial product estimand were handled through multiple imputation using the Markov Chain Monte Carlo method, assuming that non-adherent participants would have responded similarly to adherent participants within the same treatment arm

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