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Lactyl-CoA analysis and LCMS quantification Lactyl-CoA was quantified by LCMS/MS as previously described 15

Second, cells also need to be able to recycle the oxidized glutathione back to its reduced state

Li MJ, Guo TT, Lin JY, et al

Xue HG, Yang AH, Sun XG, Lu YY, Tian ZB

Briefly, 3 g of each protein sample was mixed with 15 L of 50 mM ammonium bicarbonate (pH 7.8) (Thermo Fisher Scientific), followed by protein reduction with 2 L of 100 mM dithiothreitol (Thermo Fisher Scientific) for 30 min and protein alkylation with 5 L of 2-iodoacetamide (Thermo Fisher Scientific) for an additional 30 min at room temperature in the dark

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