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Relevant fractions were concentrated and then loaded on a gel filtration column, which was equilibrated by size-exclusion chromatography (SEC) buffer (50 mM HEPES, pH 7.4, 150 mM NaCl, 5 mM MgCl 2 , and 0.5 mM tris(2-carboxyethyl)phosphine (TCEP))

This creates a hollow sphere in which active substances such as curcumin are encapsulated

Follow-up and outcomes After implementing the recommendations including SZRT, the patient reported improved sleep quality, fewer nighttime awakenings, and feeling more rested