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And their geometry properties (i.e., force field parameter) of the two customize residues were generated using antechamber package in AmberTools based on the General Amber Force Field (GAFF) 39

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Haas BJ, Papanicolaou A, Yassour M, Grabherr M, Blood PD, Bowden J, et al

Y = B max *X/(Kd + X) + NS*X + Background (X: ligand concentration, Y: total binding, B max : Maximum binding, Kd: Equilibrium dissociation constant, NS: slope of the nonlinear regression, Background: measured binding with no added ligand)

Changes in MFG numbers, therefore, are also likely to influence the concentration of MFGM components in milk