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This allows the peptide to act on the central nervous system (CNS) when applied via injection or intranasally [3]

From Figures 1A,B, the most important residue positions for M pro recognition are, in descending order, P1, P2, P1, P4, and P3, with the first 3 being the major determinants, with the minimal P2-P1 consensus being LQ/(S,A,N,G)

The following day, each 10 cm dish was transiently transfected with 3 g of the appropriate constructs as specified in the figure legends using PEI for 18 h, before being expanded into 2 10 cm dishes

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The mixture was denatured and annealed to form heteroduplexes

Yet, how lipids are delivered to LDs during their formation and growth remains a fundamental open question