glutathione elution protocol GSTrap™ Columns for Purification Removal of GST Tag by

The glutathionylated/oxidized enzymes were again purified via Ni-NTA affinitiy chromatograpghy followed by desalting on a PD-10 column as described above

doi: 10.1017/S1551929500058326

Hepatology 48(2):557566

36 Zhang C, Hu Q, Li S, Dai F, Qian W, Hewlings S, Yan T, Wang Y

isolated 20 metabolites from Scabiosa tschiliensis , using spectroscopic methods and structural characterization, including Alcohols, fatty acids, sterols, triterpenoids, flavonoids and 10 stearic acid were isolated from S

In its active form, it is made up of either two (dimer) or four (tetramer) identical subunits, each with a molecular mass of about 59-kilodaltons