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Immunohistochemistry analysis Mice in different treatment groups were anesthetized with pentobarbital sodium (Sigma-Aldrich, P-010) and perfused with PBS followed by 4% paraformaldehyde in PBS

Oocyte ATP levels The ATP level of oocytes was determined as luminescence emitted in an ATP-dependent luciferinluciferase bioluminescence assay (ATP assay kit

doi: 10.1007/s10571-023-01388-8 36 WangBWangYZhangJHuCJiangJLiYet al

Remove the plastic cap from the ipamorelin vial and wipe the rubber stopper with an alcohol swab

Addressing this question will require conditional genetic models and in vivo imaging approaches to clarify causal interactions across different stages, brain regions, and cell types