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Activity was measured by its action upon a pseudo substrate, citraconate

(Green R, et al., Nature Reviews Disease Primers, 2017 PubMed: 28660890 ) Because B12 is water-soluble, excess amounts that the body cannot use are excreted in urine rather than stored

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A., et al., R--lipoic acid does not reverse hepatic inflammation of aging, but lowers lipid anabolism, while accentuating circadian rhythm transcript profiles