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The functional activity of FVII from supernatants of iPSC-HO was assessed by two methods: a) western blotting after activation with tissue factor (TF, STA Neoplastin) and CaCl 2 and incubation with antithrombin (AT), purified from human plasma, (Grifols, Spain) and unfractionated heparin (Hospira) to measure the ability of FVII to become active and form a complex with AT

When the body is under excessive stress, these proteins exert a protective role by lessening oxidative action of the reactive oxygen species (ROS) and a wide range of metabolic stress, including structural and functional myodamage [87, 88]

Vitamin B12 absorption depends on a protein produced by the stomach called intrinsic factor

We employed Pearson correlation and simple linear regression models to investigate the correlations between potential biomarkers and clinical parameters of ALS patients (Additional file 5: Table S7)

Riboflavin levels can be assessed using a specialised measurement known as EGRAC (erythrocyte glutathione reductase activation coefficient)

A stronger immune system contributes to better overall health and well-being