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After incubation, the culture was centrifuged at 10,000 g for 10 min, and the cells were resuspended in fresh medium to reach an OD 690 of 0.4

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After 14 and 28 days, the animals were anesthetized, euthanized, and hemimandibles were collected for micro-computed tomography (micro-CT) analysis to measure lesion volume, bone volume (BV), percent of bone to total tissue volume (BV/TV), trabecular thickness (Tb.Th), trabecular number (Tb.N), and trabecular space (Tb.Sp)