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Thus, plants need to be grown in the presence of high selenium levels to accumulate meaningful amounts of methylselenocysteine

Conversely, many everyday medications are potent inhibitors of these same enzymes, creating the potential for complex, bidirectional interactions

54 These species-specific patterns reflect underlying physiological characteristics, including the cell envelope, efflux systems, and thiol metabolism

The cells were harvested by centrifugation (1000 x g , 2 min) and resuspended in lysis buffer (5% w/v 5-sulfosalicylic acid in 0.1 M potassium phosphate buffer with 5 mM EDTA (KPE buffer [35])) together with 425600 m-diameter acid-washed glass beads (Sigma) at a buffer/bead ratio of 1:2 by volume