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The membranes were blocked with TS solution [150 mM NaCl, 20 mM Tris-HCl (pH 8.0)] containing 1% non-fat dry milk for 30 min at room temperature, and then incubated with a primary antibody overnight at 4C and probed with an HRP-conjugated secondary antibody (KPL, Inc.) for 1 h at room temperature

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Eventually, the circulation and tissue concentrations equilibrate

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