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You can do this exercise twice a week

For BrdU experiments, bone marrow cells were cultured into macrophages in 100 mm dishes for two or three days, respectively, and reseeded in 6-well plates [80,000 cells/cm ( Non-adherent cells were collected, adherent cells were washed in HEPES-buffered saline solution (Promocell, Heidelberg, Germany), detached by incubating in Accutase (Sigma Aldrich) and, together with non-adherent cells, resuspended in FACS buffer before flow cytometry analysis

This will help minimize strain on the bicep tendon and promote healing

After reconstitution, refrigerate them at 28C and use within the recommended timeframe

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