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The cells were trypsinized and counted daily for up to 72 h using a Coulter counter (Beckman Coulter, USA) and TC 20 automated cell counter (BIO-RAD, #1450101J1, USA) with trypan blue

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One of the most important concepts to emerge from the field of eukaryotic cell biology is that the contents of the cell are highly organized and the movement of organelles and macromolecules within the cell does not occur through simple diffusion

While this discussion has focused on the role of GSNO reductase in mammalian cells, it is also expressed in many living organisms and has been implicated in embryonic development in amphioxus, sea squirts, and fruit flies