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SARS-CoV-2 ORF3a blocked autophagy and caused the accumulation of autophagosomes We then sought to confirm that the increase in p62 and LC3-II by ORF3a expression occurred by blocking autophagy

Studies have shown that DFO (100 M) can inhibit ROS accumulation and cell death induced by the ferroptosis inducer erastin (225)

Initially described in yeast, mitophagy was shown to be obstructed by the presence of a mutant Uth1p in the outer mitochondrial membrane (OMM) during starvation [6]

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We also used a competitive antigen-specific, T cell receptor (TCR)-dependent proliferation assay (OT-II) and a competitive homeostatic proliferation assay to assess T cell proliferation and differentiation in vivo