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The forces in each dimension are not coupled, thus the force acting on an ion in, for example, the x dimension is Here, is the quadrupolar potential, given by where is the applied electric potential and , , and are weighting factors, and is a size parameter constant

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EDTA, BD Vacutainer), which were collected into individual blood tubes

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The extracted particles were then imported into cryoSPARC and used to generate ab initio models