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The cells were fixed overnight with 75% ethanol solution at 4 C, incubated with propidiumiodide (PI) and ribonuclease (RNase) at 37 for 30 min, and then analyzed by flow cytometry (CytoFlex, Beckman Coulter)

4J-R, S2D-F)

Alignment to hg38 reference genome and gene and transcript expression levels were ran using rsem-1.3.0 48

et al., Covid-19 hyperinflammation and post-Covid-19 illness may be rooted in mast cell activation syndrome, 2020, International Journal of Infectious Diseases, Volume 100, 327-332.