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At the completion of the incubation, the cells were washed three times with ice-cold phosphate-buffered saline (PBS), and then, the fluorescence was measured by fluorescence microscopy at an emission wavelength of 460 nm using an excitation wavelength of 360 nm for the DCF fluorophore

Fisher-Wellman KH, Lin CT, Ryan TE, Reese LR, Gilliam LA, Cathey BL, Lark DS, Smith CD, Muoio DM, and Neufer PD

PLoS ONE 5 , e12520 (2010)

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