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Menon JML, Nolten C, Marijke Achterberg EJ, Joosten R, Dematteis M, Feenstra MGP, Pim Drinkenburg WH, Leenaars CHC

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neoformans Bone marrow derived macrophages (BMDMs) were isolated from BALB/c mice and differentiated at 37C with 5% CO 2 for 7 days in media consisting of DMEM, 10% FBS, 1% nonessential amino acids, 1% penicillin-streptomycin, 1% GlutaMAX (Gibco, 35050061), 1% HEPES buffer, 20% L-929 cell-conditioned supernatant, and 0.1% 2-mercaptoethanol 34