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Dietary Causes Dietary causes include too much grain and not enough roughage, consumption of moldy feedstuffs, diets high in sulfur, cobalt-deficient diets, or consuming certain toxic plants such as bracken ferns

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to an OD 600 of 0.7-0.8 (2-3 h) before induction with 0.5 mM IPTG and incubated for an additional 3 h

In many cases, switching from oral to intramuscular B12 circumvents absorption issues entirely, providing reliable therapeutic levels regardless of gastrointestinal factors or drug interactions

Patients randomized to placebo, pemetrexed, and platinum chemotherapy were offered pembrolizumab as a single agent at the time of disease progression