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Real timePCR Total RNA of the cells was extracted using TRIzol reagent (Invitrogen), the RNA was converted to cDNA and the RTPCR was performed using NGF gene-specific primers (Supplementary Table 2) and the 7900 Fast RTPCR system (Applied Biosystems) to determine NGF mRNA expression

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10.1016/j.jconrel.2016.01.006 52 KatoK.ChinK.YoshikawaT.YamaguchiK.TsujiY.EsakiT.et al (2012)