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36 , e22496 (2022)

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MTT Assay Normal and vitiligo melanocytes, treated with the autophagy inhibitor 3-methyl adenine as indicated, were then incubated with 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) (1 mg/mL) for 2 h at 37 C, and lysed in dimethyl sulfoxide (DMSO)

GSH, a tripeptide of glutamine, cysteine, and glycine, is the most abundant antioxidant capable of providing reducing equivalents and also serves as a versatile nucleophilic cofactor in a wide spectrum of metabolic reactions in aerobic organisms (63, 64)

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The predicted C max and AUC ratios were highest in G-EM, followed by G-IM and G-PM, consistent with the expected gradient of CYP2C19 metabolic activity across phenotypes