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Studies in humans have shown that spermidine can improve cognitive decline in senior citizens at risk of dementia (Wirth et al., 2018) and is associated with a reduced risk of CV diseases ( The average daily spermidine intake is 725 mg or more, with the highest reported levels found in the MedDiet ( 4.3 Alpha-ketoglutarate Alpha-ketoglutarate (AKG) is a molecule naturally present in our cells

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Based on these results, 12 C of induction temperature, 0.2 mM of IPTG concentration and 24 h of induction time were determined as the optimal induction conditions, which could guarantee the maximum soluble expression of fusion protein TAT-CcSOD1