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This subsequently initiates an endothelium-to-mesenchyme transition characterised by progressive loss of vascular endothelial markers (VE-cadherin, CD31), synchronous acquisition of interstitial markers (-smooth muscle actin, fibronectin), and excessive extracellular matrix deposition [17, 44]

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From these animals, we isolated the caudal brainstem containing the DVC and performed single-nucleus RNA sequencing (snRNA-seq