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After treatment (100 M Hcy, 24 h), the medium was changed to unbuffered XF assay medium (103793, Agilent Technologies, USA) supplemented with 5 mM glucose, 1 mM pyruvate, and 2 mM glutamine and incubated in a CO 2 -free incubator at 37 C for 1 h

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These tissues can sometimes be slow to recover, particularly when blood flow is limited

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The World Anti-Doping Agency prohibits them, and the NCAA and the U.S