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Then, cells were centrifuged and resuspended into 2 mL of prewarmed hypotonic solution (KCl 0.075 mmol L 1 ) and incubated in 14 mL of this solution for 15 min at 37 C

Salt Sensing by Serum/Glucocorticoid-Regulated Kinase 1 Promotes Th17-like Inflammatory Adaptation of Foxp3(+) Regulatory T Cells

However, the digestible carbohydrate content and composition of the cocoa pod (pulp and beans) are significantly changed after six or more days of bacterial fermentation, as per the conventional method of processing cacao beans (Obinze, Ojimelukwe, & Eke, 2022)

- Glycerol used in the freezing medium was stored in light (if applicable): If stored in light, glycerol gets converted into acrolein, which is toxic to cells