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Activation of AMP-activated protein kinase is involved in vincristine-induced cell apoptosis in B16 melanoma cell

Cellular Immunofluorescence assay Cells were fixed in 4% paraformaldehyde for 15 min at room temperature, washed thoroughly in PBS, and permeabilized with 0.1% Triton X-100 for 12 min

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In essence, MOTS-c acts as a metabolic master switch that promotes energy homeostasis: it helps cells take up and use glucose, ramps up fat burning, and avoids metabolic dysfunction under stress conditions