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To determine whether GSH related proteins linked to NFR2 regulated transcription showed time of day differences in their expression patterns, we examined the expression of Glutamate-Cysteine Ligase (GCLC) -the enzyme involved in the first step of GSH synthesis, Glutathione Synthetase (GS) - the enzyme involved in the second step of GSH synthesis, and Glutathione Reductase (GR) -the enzyme involved in the regeneration of GSH ( Figures 4BD )

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Accumulated lipids also induce mitochondrial fission and apoptosis in renal cells, suggesting that consumption of an HFD causes renal cell injury, which may be attributed to oxidative stress and mitochondrial dysfunction, promoting excess programmed cell death [172]