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Briefly, cells were maintained in growth medium (High-glucose Dulbeccos modified Eagles medium (DMEM) supplemented with 10% FBS, 2 mM L-glutamine, 100 U/ml penicillin, 100 mg/ml streptomycin (all from Thermo Fisher Scientific Waltham, MA, United States))

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It seems, that DSIP passes the blood-brain barrier more easily than many other neuropeptides

The interaction of these neurons, specifically the lateral parabrachial nucleus pathway, needs to be further elucidated in future studies

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