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10.1007/s44307-024-00031-x 253 ZhaoX.XuS.LiuC.HeJ.LiC.DengY.et al (2020)

10.1016/j.vph.2018.02.010 76 VukojevicJ.VrdoljakB.MalekinusicD.SiroglavicM.MilavicM.KolencD.et al (2020)

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GEMMS involve targeted modifications of the murine genome, such as gene knockout (deletion), knock-in (mutagenesis), or transgenic overexpression, enabling the study of tumor biology within an intact immune system and native tissue microenvironment

The relative expression of target genes was calculated using the 2e(-Ct) method ( ELISA To access the intracellular protein levels of CCL3 and IL-1B, cell precipitates of aliquots from endpoints A and B were homogenized in 1X PBS, diluted in Milli-Q H 2 O 1:2 and the cells disrupted by mechanical lysis in the TissueLyser (Qiagen) for 2 minutes at 30 Hz and three freeze/thaw cycles (20 to -20C)

That is, when glutamate binds to the AMPA receptor, the shape of the receptor protein changes, opening a channel for Na + ions