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The five internal controls were calculated on their geometric mean for the normalization of RT-qPCR data (Sumby et al., 2012)

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We thank the Advanced Imaging Facility (RRID:SCR_020967) at the University of Leicester for their support

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Based on these data, we have determined the following proposed applicable percentage increases to the standardized amount for FY 2024 for Puerto Rico hospitals: For a Puerto Rico hospital that is a meaningful EHR user, we are proposing a FY 2024 applicable percentage increase to the operating standardized amount of 2.8 percent (that is, the FY 2024 estimate of the proposed market basket rate-of-increase of 3.0 percent less 0.2 percentage point for the proposed productivity adjustment)

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