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The fact that protein cyclization is seldom used for protein optimization cannot be ascribed to inappropriate NC distances, because half of the protein domains found in the protein data bank (PDB) have their C and N extremities joinable by linkers made of a few amino acids 40

Wang L, Tang J, Wang L, et al

The tissue becomes sensitized, developing localized immune memory that triggers faster and stronger responses with each subsequent injection

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In this model, male Wistar rats were subjected to either WA or sham WA for 1-h daily, 10 days sequentially