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Measurement of encapsulated Mg 2+ concentration The internal Mg 2+ concentration of the vesicles was measured using mag-fura-2 according to the procedures described earlier 39

The lysate was cleared by centrifugation and the protein was purified by Ni-His-affinity chromatography in batch mode

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Similar content being viewed by others Log in or create a free account to read this content Gain free access to this article, as well as selected content from this journal and more on nature.com or Abbreviations E-GPx: extracellular glutathione peroxidase C-GPx: cellular glutathione peroxidase GPx-GI: gastrointestinal glutathione peroxidase GPx: seleniumdependent glutathione peroxidase IBD: inflammatory bowel disease DSS: dextran sodium sulfate GI: gastrointestinal t-BuOOH: tert-butyl hydroperoxide GSH: glutathione SSC: saline-sodium citrate GAPD: glyceraldehyde-3 phosphate dehydrogenase References Parks DA, Bulkley GB, Granger DN 1983 Role of oxygen-derived free radicals in digestive tract diseases

Canto P, Vilchis F, Soderlund D, Reyes E, Mendez JP: A heterozygous mutation in the desert hedgehog gene in patients with mixed gonadal dysgenesis

PMID: 12522009