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Relying solely on a single pathway limits a providers ability to treat complex metabolic resistance

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Transwell assay The chemotactic motility of HUVECs was determined using transwell migration chambers (Corning) with 6.5 mm diameter polycarbonate filters (8 m pore size), as described previously.28 In brief, the bottom chambers were filled with 750 mL of RPMI 1640 medium containing all supplements

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The purpose of this study was to illustrate how challenging it is to design a peptide-based cancer vaccine and to balance delivery method, half-life, epitope selection, and immunogenicity to produce a successful vaccine strategy