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The strategy adopted is reported in a recent paper (72)

A small amount of vaccine enters the arm muscle, and the immune system reacts with warmth, swelling and soreness that tighten the muscle fibers

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The authors found that a 14 day course of treatment with metronidazole given to healthy dogs, resulted in significant changes in microbiome composition, including severely decreased amounts of a key beneficial bacteria, Fusobacteria

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