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Liposomal Glutathione Liposomal formulations are designed to enhance bioavailability

Construction of NQO1 mutant cell lines HEK293T cells were transfected with a plasmid encoding Cas9-NLS, an NQO1-targeted sgRNA (target region, 5-CCATCTGAGCCCAGATATTG-3), and each of the HDR templates (S82D, GCTTATAAAGAAGGCCATCTGGACCCAGATATCGTTGCTGAACAAAAGAAG

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The exact reconstitution calculations

Mitolysosome quantification was performed on Pimo-positive cells as described under Mitolysosome Quantification (below), with the exception that the blue channel analyzed was Pimo, not RBPMS