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Photographer Yi-Chin Le and I agreed early on that we would not take any risks driving into water, but it turned out that her idea of "not a risk" was a bit different from mine

Understanding autism through this biological framework opens the door to new therapeutic strategies particularly those focused on reducing inflammation, improving mitochondrial function, and restoring microbiome balance

Then cDNA was synthesized using High-Capacity cDNA Reverse Transcription Kits (4374967, Applied Biosystems) with the accompanied random primers, as described previously ( The mRNA expression levels of given genes in the transfected cells with or without cell sorting enrichment were determined by qPCR using Taq388 mix (Du et al., 2022) on a QIAGEN Q-Rex machine (Qiagen, Germany)

Subsequently, the cells were seeded in a 6-well plate at a density of 3 10 6 cells per well

C-type cells are intermediate transient neuronal cells (nIPC) that proliferate rapidly and differentiate into neuroblasts or type-A cells (Nualart et al., 2012