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The precise molecular identity of these binding sites remains under active investigation, but their characterisation has strengthened the mechanistic basis for Semax's CNS activity and supported its classification as a compound with defined receptor-level pharmacology rather than merely non-specific neuromodulatory effects

Mitochondrial potential loss was measured using the Varioskan kinetic plate reader at excitation 545 nm and emission 590 nm for 3 h at 21 C (kinetic measurements every 5 min)

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