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45 min prior to the assay, cells were washed and placed in KHB medium supplemented with 2.5 mM glucose, 0.5 mM L-carnitine, and 5 mM Hepes (12509079, Gibco), pH 7.4 in a CO 2 -free incubator at 37 C

Relating inflammatory markers in patients to clinical symptoms has yielded some insight (Jonsj et al., 2020)

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Among them was Ethiopian legend Kenenisa Bekele, who owned the Olympic record for the distance at 27 minutes and 1.17 seconds and the world record of 26 minutes and 17.53 seconds, and had won the previous two Olympic gold medals in the event