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For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample

Briefly, a standard curve was generated using known concentrations of bovine serum albumin (Sigma) and used to calculate sample concentrations using a Varioskan Flash plate reader (Thermo Scientific)

Zhao Y, Zhang Y, Kong H, Cheng G, Qu H, Zhao Y (2022) Protective effects of carbon dots derived from Armeniacae semen amarum carbonisata against acute lung injury induced by lipopolysaccharides in rats

Jackson and Nellen, 1966

The first day I took it I felt energized- Ive started walking again

Animal care and all experimental procedures were performed in accordance with the Guideline for Animal Experimentation of Nagasaki University with the approval of the Institutional Animal Care and Use Committee (permission number: 1803191437)