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As male mice more robustly develop symptoms of metabolic syndrome than female, only male Ctrl and KO mice were challenged with a 60% high fat diet (D12492, HFD, Research Diets, New Brunswick, USA) for 12 weeks, starting at the age of 5 weeks, before p53 ablation was facilitated by treating the mice with Tamoxifen (100 mg/kg via oral gavage for 5 consecutive days (Molekula group, Munich, Germany) followed by a 1 week wash out period

There are some mechanisms involved in GSH diminution: increased oxidation by intracellular oxidizing agents, increased conjugation to molecules, and increased exit across the cell membrane [137]

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[DOI] [PMC free article] [PubMed] [Google Scholar] 185.KAZAK L, COHEN P

However, a simple increase in payload conjugation at high DAR can lead to conjugate hydrophobicity, leading to faster clearance by the mononuclear phagocyte system and accelerated plasma elimination, rather than renal clearance which is predominant for small molecules [52]