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A BCA protein assay kit (Beyotime, China) was used to quantify the protein concentration, and 15-30 g of each sample was separated on 8%-12% SDS-PAGE gels (Beyotime, China)

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The vertical dotted line corresponds to the average z-normalized LFC of the controls (intergenic and non-targeting) minus 1x standard deviation of the entire genetic screen

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Conclusion: The IDR target site is highly conserved in baseline samples from CHB subjects enrolled in IDR clinical studies assessed to date

coli indicates that they can mutagenically acquire, but do not constitutively possess, an alternative disulfide reductase system