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Briefly, when HepG2 cells reached 7080% confluence, solutions A (2.5 g plasmid DNA, 2.5 L PLUS reagent, and 125 L Opti-MEM) and solution B (5 L LTX reagent and 125 L Opti-MEM) were prepared separately, combined, and incubated for 5 min before being added dropwise to the cell culture medium

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Two screening methods and hCG detection methods (intact and tryptically digested) were applied

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