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To denature the protein, it was exposed to a temperature of 100 C water bath lasts 10 min

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6 Tamoxifen-induced toxicity associated with oxidative stress and its prevention Tamoxifen stimulates the generation of oxidative stress that can result in the damage of cellular structures [100]

Of note, whereas the 485 SFQVI 489 motif contained a canonical LIR sequence, the 413 FAECV 417 motif bore cysteine substituted for the L/I/V residue specified by the canonical four-residue LIR motif

Antioxidant effects after coffee enema or oral coffee consumption in healthy Thai male volunteers S Teekachunhatean N Tosri C Sangdee R Wongpoomchai W Ruangyuttikarn C Puaninta S Srichairatanakool We designed an open-label, randomized two-phase crossover study to investigate the antioxidant effects after single and multiple doses of a coffee enema versus coffee consumed orally