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A target protein can be separated from other molecules via specific properties like molecular mass, charge, hydrophobicity or affinity to another molecule, whereas the latter the most efficient property is

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The moisture was restored by adding distilled water and the spiked sediment was carried out using stock solutions of each metal of interest, prepared by the dissolution of inorganic salts of copper sulfate (CuSO 4 ), lead chloride (PbCl 2 ), zinc sulfate (ZnSO 4 ), and mercuric chloride (HgCl 2 ) (Merck , reagent grade) in distilled water, following the protocols established by the United States Environmental Protection Agency (United States Environmental Protection Agency (USEPA), 2001) and the American Society for Testing and Materials (American Society for Testing Materials (ASTM), 1 of Cu, 22.65 mg kg 1 of Pb, 93.0 mg kg 1 of Zn, and 0.1 mg kg 1 of Hg