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TGP-free tt SI was then released from the column by His-tag-free 3C protease digestion, concentrated using a 100-kDa cutoff filtration membrane, heated at various temperatures, and used for FSEC-TS assay with the same setup as for GFP fusion protein except that the excitation and emission wavelength pair was changed to 280/350 nm

Selective disruption of mitochondrial thiol redox state in cells and in vivo

As expected from the coupling of osteoblast-osteoclast functions, the loss of IGF-1 in the osteoblast also negatively affects osteoclast proliferation

For COs, over 20 mitochondrial-related metabolites were quantified using liquid chromatography mass spectrometry (LC-MS)

Such upregulation most frequently occurs via loss-of-function mutations in KEAP1, the E3 ubiquitin ligase that degrades NRF2

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