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Inflammopharmacology 29 , 17691776 (2021)

After centrifugation for 10 min at 12,000 g at 4 C, the supernatants were collected and prewashed by adding protein A/G agarose (Pierce, 20421) before incubation for 2 h at 4 C

Acetyl glutathione penetrates the mitochondrial membranes, helping to maintain their integrity and function, and crosses the blood-brain barrier, allowing it to directly detoxify and protect the brain